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anti zo2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti zo2
    Anti Zo2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+zo2/ZO-2+Antibody/pm41634022-347-14-16
    Average 94 stars, based on 105 article reviews
    anti zo2 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: In vivo dual RNA-Seq uncovers key effectors of epithelial barrier disruption by an extracellular pathogen
    Article Snippet: 10 μg of total proteins were loaded on a 12% SDS-PAGE (Mini-PROTEAN TGX Stain-Free precast gels, Biorad) and transferred onto nitrocellulose membrane. .. The membranes were blocked with PBS Tween20 0.1% containing 5% BSA for 1 h at RT and incubated overnight at 4 °C with the antibodies anti-ZO1 (13663; Cell Signaling), anti-ZO2 (2847; Cell Signaling), anti-Profilin-1 (3246; Cell Signaling), anti-WAVE-2 (3659; Cell Signaling), anti-pRac1 (2461; Cell Signaling), anti-pCofilin (3313; Cell Signaling), anti-Cofilin (5175; Cell Signaling), anti-GAPDH (5174; Cell Signaling), anti-MLC2 (8505; Cell Signaling), anti-pMLC2 (3671; Cell Signaling), anti-PERK (3192; Cell Signaling), anti-pPERK (PA5-40294; ThermoFisher), anti-IRE1 (3294; Cell Signaling), anti-pIRE1 (PA1-16927; ThermoFisher) and anti-3xFLAG (87537; Cell Signaling) at a 1:1000 dilution. .. Membranes were washed in PBS-0.1% Tween20 and incubated at RT with secondary HRP-conjugated antibody (7074S; Cell Signaling) at a 1:1000 dilution for 1 h. Detection was performed with SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher).

    Article Title: In vivo dual RNA-Seq uncovers key effectors of epithelial barrier disruption by an extracellular pathogen.
    Article Snippet: At the indicated times, the cells were fixed with 4% paraformaldehyde for 15min at room temperature (RT) and subsequently incubated for 10min in 0.5% saponin (Sigma) in PBS and for 1 h in 1% BSA (Sigma) and 0.075% saponin in PBS. .. The cells were incubated overnight at 4 °C with the anti-ZO1 (13663; Cell Signaling), anti-ZO2 (2847; Cell Signaling), antiJAM1 (82196; Cell Signaling), anti-pan-cadherin (PA5-16766; ThermoFisher) or anti-connexin-43 (3512; Cell Signaling) antibodies at 1:100. .. Cells were washed and incubated for 1 h with Alexa Fluor 555 or 488 antibody (ThermoFisher) at 1:500.

    Article Title: In vivo dual RNA-Seq uncovers key effectors of epithelial barrier disruption by an extracellular pathogen
    Article Snippet: At the indicated times, the cells were fixed with 4% paraformaldehyde for 15 min at room temperature (RT) and subsequently incubated for 10 min in 0.5% saponin (Sigma) in PBS and for 1 h in 1% BSA (Sigma) and 0.075% saponin in PBS. .. The cells were incubated overnight at 4 °C with the anti-ZO1 (13663; Cell Signaling), anti-ZO2 (2847; Cell Signaling), anti-JAM1 (82196; Cell Signaling), anti-pan-cadherin (PA5-16766; ThermoFisher) or anti-connexin-43 (3512; Cell Signaling) antibodies at 1:100. .. Cells were washed and incubated for 1 h with Alexa Fluor 555 or 488 antibody (ThermoFisher) at 1:500.

    Article Title: In Vivo Dual RNA-Seq uncovers key toxin-like effectors of epithelial barrier disruption and tissue colonization by an extracellular bacterial pathogen
    Article Snippet: At the indicated times, the cells were fixed with 4% paraformaldehyde for 15 min at room temperature (RT) and subsequently incubated for 10 min in 0.5% saponin (Sigma) in PBS and for 1 hr in 1% BSA (Sigma) and 0.075% saponin in PBS. .. The cells were incubated overnight at 4°C with the anti-ZO1 (13663; Cell Signaling), anti-ZO2 (2847; Cell Signaling), anti-F-actin (PHDH1; Tebu), anti-JAM1 (82196; Cell Signaling) or anti-connexin-43 (3512; Cell Signaling) antibodies at 1:100. .. Cells were washed and incubated for 1 hr with Alexa Fluor 555 or 488 antibody (ThermoFisher) at 1:500.

    Article Title: In vivo dual RNA-Seq uncovers key effectors of epithelial barrier disruption by an extracellular pathogen.
    Article Snippet: 10 μg of total proteins were loaded on a 12% SDS-PAGE (Mini-PROTEAN TGX Stain-Free precast gels, Biorad) and transferred onto nitrocellulose membrane. .. The membranes were blocked with PBS Tween20 0.1% containing 5% BSA for 1 h at RT and incubated overnight at 4 °C with the antibodies anti-ZO1 (13663; Cell Signaling), anti-ZO2 (2847; Cell Signaling), anti-Profilin-1 (3246; Cell Signaling), anti-WAVE-2 (3659; Cell Signaling), anti-pRac1 (2461; Cell Signaling), anti-pCofilin (3313; Cell Signaling), anti-Cofilin (5175; Cell Signaling), anti-GAPDH (5174; Cell Signaling), anti-MLC2 (8505; Cell Signaling), anti-pMLC2 (3671; Cell Signaling), anti-PERK (3192; Cell Signaling), anti-pPERK (PA5-40294; ThermoFisher), anti-IRE1 (3294; Cell Signaling), anti-pIRE1 (PA1-16927; ThermoFisher) and anti-3xFLAG (87537; Cell Signaling) at a 1:1000 dilution.Membranes werewashed in PBS-0.1% Tween20 and incubated at RT with secondary HRPconjugated antibody (7074S; Cell Signaling) at a 1:1000 dilution for 1 h. Detection was performed with SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher). ..

    Article Title: In Vivo Dual RNA-Seq uncovers key toxin-like effectors of epithelial barrier disruption and tissue colonization by an extracellular bacterial pathogen
    Article Snippet: .. The membranes were blocked with PBS Tween20 0.1% containing 5% BSA for 1 hr at RT and incubated overnight at 4°C with the antibodies anti-ZO1 (13663; Cell Signaling), anti-ZO2 (2847; Cell Signaling), anti-Profilin-1 (3246; Cell Signaling), anti-WAVE-2 (3659; Cell Signaling), anti-pRac1 (2461; Cell Signaling), anti-pCofilin (3313; Cell Signaling), anti-Cofilin (5175; Cell Signaling), anti-GAPDH (5174; Cell Signaling), anti-MLC2 (8505; Cell Signaling), anti-pMLC2 (3671; Cell Signaling), anti-PERK (3192; Cell Signaling), anti-pPERK (PA5-40294; ThermoFisher), anti-IRE1 (3294; Cell Signaling), anti-pIRE1 (PA1-16927; ThermoFisher) and anti-3xFLAG (87537; Cell Signaling) at a 1:1000 dilution. .. Membranes were washed in PBS-0.1% Tween20 and incubated at RT with secondary HRP-conjugated antibody (7074S; Cell Signaling) at a 1:1000 dilution for 1 hr.

    other:

    Article Title: The Host Interactome of Spike Expands the Tropism of SARS-CoV-2
    Article Snippet: The plasma membrane was stained with CF594 dye conjugated wheat germ agglutinin (Biotium), while ER was stained with the ER Cytopainter staining kit (AbCam) according to manufacturer recommendations.

    Transferring:

    Article Title: The Host Interactome of Spike Expands the Tropism of SARS-CoV-2
    Article Snippet: Images were taken with a Zeiss LSM 780 confocal laser scanning microscope and analyzed using Fiji/ImageJ (v.2.1.0/1.53c). .. One volume of 2x SDS sample loading buffer was added to samples in TN buffer (1:1; v:v) and samples were heated to 50°C for 15 min. Proteins were separated on NuPAGE 4-12% Bis-Tris gels (Life technologies) before transferring proteins onto Nitrocellulose membranes and incubating with anti-BiP (C50B12, Cell Signaling Laboratories (CSL)), anti-ZO1 (D7D12, CSL), anti-ZO2 (2847, CSL) or Alexa Fluor 488 conjugated anti-DYKDDDDK Tag monoclonal antibody (L5, Sigma) in 5 % BSA, 1x PBS, 0.05 % Tween-20. .. Signals were detected either using Radiance Q ECL reagent (Azure Biosystems) after washing and incubation with HRP coupled anti-rabbit antibodies (CSL) or imaged directly using an Azure 600 Western blot imaging system.



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